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31759

Genome-wide DNA Mutability and Biochemical Effects of Novel Insecticides in the Control of Date Palm Fruit Pest Ephestia cautella (Walker)

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Last updated: 01 Jan 2025

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Abstract

Different pests attack date palm fruits during all the stages of maturity. Almond moth (Ephestia cautella) is a destructive insect of stored products, such as dates, wheat flour, and nuts. In the present study, the usage of novel groups of insecticides (Spinosyns, Diamide, Pyridalyl, and Azadirachtin) that are safe for humans and exert a relatively lower effect on the environment was assessed. In particular, the toxicological and biochemical impacts of five insecticides (Tracer, Radiant, Coragen, Pleo, and Achook) on the control of E. Cautella were evaluated, and the genome-wide DNA mutability caused by these insecticides was screened. A bioassay was performed in order to determine the LC50value for each insecticide, the results of which revealed that Coragen with an LC50 value of 0.49 ppm was the most potent insecticide, followed by Radiant and Achook (with LC50 values of 1.51 and 1.73 ppm, respectively). In addition, the effect of these insecticides on vital enzymes was investigated. The data from this investigation revealed that the treatment with Radiant demonstrated stimulation in AST, ACP, ALP, and GST activity, and inhibition in AChE, ALT, and protease activity. The insects treated with Coragen exhibited an elevation in the activities of AChE, GST, ALP, ACP, ALT, and protease. Finally, DNA-level mutability caused by the insecticides was assessed by using RAPD-PCR analysis, and the results indicated Radiant as a genotoxic insecticide that caused large changes at the genomic-DNA level. On the other hand, Coragen exhibited the lowest mutability effect on insect DNA.

DOI

10.21608/eajbsf.2019.31759

Keywords

Toxicological, Ephestia cautella. Insecticides. RAPD. Date palm

Authors

First Name

Fouad

Last Name

Ahmed

MiddleName

A.

Affiliation

Biochemistry Department, Faculty of Agricultural, Cairo University, Egypt

Email

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City

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Orcid

-

First Name

Mohamed

Last Name

Rashed

MiddleName

M.

Affiliation

Biochemistry Department, Faculty of Agricultural, Cairo University, Egypt

Email

-

City

-

Orcid

-

First Name

Hala

Last Name

Abou-Yousf

MiddleName

M.

Affiliation

BioassayDepartment, Central Agricultural Pesticide Laboratory (CAPL), Agriculture Research Center (ARC). Egypt

Email

-

City

-

Orcid

-

First Name

Emam

Last Name

Abdel-Rahim

MiddleName

A.

Affiliation

Biochemistry Department, Faculty of Agricultural, Cairo University, Egypt

Email

-

City

-

Orcid

-

First Name

Shaimaa

Last Name

Mahdi

MiddleName

M.

Affiliation

BioassayDepartment, Central Agricultural Pesticide Laboratory (CAPL), Agriculture Research Center (ARC). Egypt.

Email

-

City

-

Orcid

-

First Name

Gamal

Last Name

Osman

MiddleName

H.

Affiliation

-Biology Department, Faculty of Applied Sciences, Umm Al-Qura University, Makkah, 21955, PO Box 715, Saudi Arabia. - Microbial Genetics Lab., Agriculture Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC). Egypt.

Email

geosman@uqu.edu.sa

City

-

Orcid

-

First Name

Mohamed

Last Name

Atia

MiddleName

A.M.

Affiliation

Molecular Genetics and Genome Mapping Lab., Agriculture Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC). Egypt

Email

matia@ageri.sci.eg

City

-

Orcid

-

Volume

11

Article Issue

1

Related Issue

4594

Issue Date

2019-01-01

Receive Date

2019-02-03

Publish Date

2019-01-01

Page Start

105

Page End

22

Print ISSN

2090-0864

Online ISSN

2090-0791

Article File

EAJBSF_Volume 11_Issue 1_Pages 105-22.pdf

PDF . 1.4MB

Link

https://eajbsf.journals.ekb.eg/article_31759.html

Detail API

https://eajbsf.journals.ekb.eg/service?article_code=31759

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Original Article

Type Code

6

Publication Type

Journal

Publication Title

Egyptian Academic Journal of Biological Sciences, F. Toxicology & Pest Control

Publication Link

https://eajbsf.journals.ekb.eg/

MainTitle

Genome-wide DNA Mutability and Biochemical Effects of Novel Insecticides in the Control of Date Palm Fruit Pest Ephestia cautella (Walker)

Details

Type

Article

Created At

22 Jan 2023