Curassavine was isolated as a yellowish viscous liquid and identified as (ester of trachelanthamidine and 3-carboxy-4-methylhexane-2,3-diol) by the aid of mass spectrometry (MS) and Nuclear Magnetic Resonance spectroscopy (¹H and ¹³C-NMR).Considerable mortality was evident after the treatment. With regard to the larvicidal activity, the obtained data showed mortality percentages between 4 and 24 % using concentrations between 100 and 500 mg/ml after 3 days. The mortality increased to reach 8 and 86 % for the same concentrations within 5 days. LC₅₀ was 288.4 mg/ml. Concerning the pupicidal activity, no effect using concentrations between 100 and 500 mg/ml within 2 days; while they showed mortality percentages between 8 and    60 % within 5 days. LC₅₀ was 512.9 mg/ml. On the other hand, Curassavine caused mortality percentages among larvae and pupae of Culex  pipiens between 12 and 84 % using concentrations between 100 and 500 µg/ml after one day. While they showed mortality percentages between 16 and 100 % within 2 days; Value of LC₅₀ was 147.9 µg/ml.