Olive tree is subjected to attack by many insect pest species that effect on the yield quality and quantity. Among the most common pest species surveyed in Egypt is; the leopard Zeuzera pyrina (L.) (Lepidoptera: Cossidae), which considered a serious pest in olive fields causing a lot of damage and loss in olive trees. Imidacloprid is one of the natural insecticide cause the infestations decrease of many insect pests. The effect of Imidacloprid was tested under laboratory and field conditions against Z. pyrina.  Results showed that the LC₅₀ of Imidacloprid  recoded 120 ppm when Z. pyrina treated with different concentrations. When the nano imidaclorprid applied on the target pests the LC₅₀ recorded 47 ppm. Under field conditions, the infestations were significantly decreased to 23±8.9 and13±2.1 individuals after treated with Imidacloprid in Ebn Malek and Ismailia, respectively.  In the same last places the nano Imidacloprid application showed a significant decrease in the pests infestations reached to 15±5.1 and 6±6.6 larvae as compared to 95±1.9 and 96±3.4 larvae in the control. The yields weights in both two regions were significantly increased as the result of nano imidacloprid applications.