Seventy three pure actinomycete colonies were isolated from 48rhizospheric soil samples revealing different locations in Sinai. These isolates were subjected for measurement of their insecticidal activities against the greater wax moth Galleria mellonella L. of them seven isolates (S6, S13, S16, S23, S27, S35 and S36) were found as the most potent and were chosen for detailed toxicological studies. Their LC₅₀ values were 25.23, 36.80, 55.96, 52.02, 54.19, 54.52 and 32.88 mg/ml, respectively. The most potent isolate (S6) was isolated from the rhizosphere of Tamarix nilatie plants grown in a sandy soil at El-Tor area and taxonomically was identified as Streptomyces lavendulae.