The two-spotted spider mite, Tetranychus urticae (Koch) is one of the major pest attacking different field crops, vegetables and fruits, while the predatory mite Phytoseiulus persimilis (Athias-Henriot) is well known as predator specialized on tetranychida mites. Nanotechnology is science of manipulating materials at nano-scale. Among the latest technological advancements. Nanotechnology and common solution of some compounds occupies a major position in pest control. So, the present investigation was carried out to evaluate some compounds (Cyhalothrin 5% SC, Abamectin Benzoate 1.8 % SC,Chlorpyrifos 48% SC and Methomyl 95 % WP) and their images Nan particles against T. urticae and P. persimilis in laboratory and under field conductions on cotton plants. Also, the LC50 of these chemicals on the mite and predatory mite were evaluated .The results revealed that Abamectin Benzoate nano particles had high toxic effect and high toxicity index , on the other hand Methomyl  95 nano partials was least toxic compounds to adult female T. urticae than the tested compounds in common solution. Moreover Cyhalothrin nano partial was the most effective compound against T. urticae eggs deposition and eggs hatchability of T. urticae. But Cyhalothrin nano partials had moderate effect on mite eggs and adults of T. urticae.Under field condition, all compounds of nano particles achieved good effect against T. urticae on cotton plants. But all compounds were safety to predatory mite P.persimilis in comparison with T. urticae. The current investigation could be recommended as an aspect of integrated pest management against T. urticae and P. persimilis both in the field.