Bioinsecticides considered a one of the safest method for pest control. A field study was conducted to compare the efficiency of the two types of bioinsecticides with two different mode of action against Egyptian cotton leaf worm Spodoptera littoralis (Boisd.). The recommended rate of these insecticides was sprayed on the foliage of cotton plants in the field and the reduction % were recorded after 2,4,6,8 days. Also, semi field studies were conducted to determine the latent effects of tested bioinsecticides on biological aspects. The results revealed that Spinetoram filed treatment recorded the highest reduction present  95.5% after 2 days and reduced gradually to 74.0% after 8 days with general mean 85.1%, while Dipel 2X, caused 24.8% reduction percent after 2 days and increased gradually up to 86.7 % after 8 days with general mean 55.9 %. Semi filed studied explained that; Spinetoram treatment was more effective on 2^nd and 4^th S. littoralis biological aspects than Dipel 2X treatment, specially; larval mortality, pupal mortality, adult emergence, malformed adult % fecundity and fertility. These results indicated that Spinetoram had the potentiality to be promising substitutes of conventional toxicants for S. littoralis control under field conditions.