The effect of Methoxyfenzoid, Indoxacarb and Emmamectin Benzoate on Carbohydrate and Phosphatase Enzymes of field collected Tuta Absoluta was investigated. Toxicity results against third instar larvae showed LC₅₀ values was (50.77 ppm, 2.33 ppm and 0.22 ppm) for Methoxyfenzoid, Indoxacarb and Emmamectin Benzoate respectively. Additional results, indoxacarb and emmamectin benzoate are faster to kill than methoxyfenzoid where LT₅₀ was (1420.8 min 1469.9 min and 2629.3 min) respectively. The biochemical results showed high increase in trehalase and acid phosphatase (17.93, 7.83%) respectively and slight increase in invertase and amylase activities (2.51 and 0.039%) respectively when treated with indoxacarb comparing the other two insecticide.