Background and Aim: Chronic infection with hepatitis C virus (HCV) is a huge problem both globally and at the level of the individual patient. The natural outcome and response to treatment in hepatitis C virus (HCV) infection varies between individuals. We aimed to detect single nucleotide polymorphisms (SNPs) of the IL-I0 promoter region at nucleotides -1082 &-592 and IL-12 promotor region at nucleotide -1188., also we measured Il-10 and IL12 protein level in the serum of chronic liver diseased patients under treatment with peg IFN- plus ribavirin and compare the results with non infected subjects. Subject and Methods: The study included 80 patients with chronic hepatitis C received PEG-IFN alpha-2b plus ribavirin for 24 weeks and 100 healthy subjects serving as control. The following was done; Liver function tests, hepatitis markers, HCV quantitation by real time PCR, DNA extraction from whole blood, PCR RFLP and quantitation of protein level of IL-10 and IL-12 by ELISA. Results: The serum levels of IL-10 were significantly decreased; in contrast, the serum levels of Il-12 were significantly increased in responder compared with non responder (p= 0.000). Single nucleotide polymorphism (SNP) in the promotor region of the IL-10 gene (IL-10) at nucleotide (nt) -1082 shows significant difference regard genotypes (AA and AG), where AG genotype had greater frequency in responders than AA (63.8 %, 10.6 %) respectively while no significant difference was found regard genotype GG among responders and non responders. The subjects with G allele showed higher rates of response than those with A allele. IL-10 -592 showed significant difference in its genotype frequencies between responders and non-responders regard (AA and CC) where AA genotype had greater frequency in responders than CC (42.6 %, 36.2 %) respectively. Meanwhile, no significant difference was found regard genotype (AC) among responders and non responders. The subjects with A allele showed higher rates of response than those with C allele. IL-12 -1188 showed significant difference in its genotype frequencies between responders and non-responders regard (AA) genotype. While, no significant difference regard genotype (AC and CC) among responders and non responders. The subjects with A allele showed higher rates of response than those with C allele.Conclusion: It can be concluded that SNPs in IL-10 and IL-12 and their serum levels may be promising predictors for HCV therapy.