The retinalnerve fiber layer (RNFL) is formed by the expansion of the fibers of the optic nerve; it is thickest near the optic disc, gradually diminishing toward the oraserrata. Most of the fibers are centripetal, and are the direct continuations of the axis-cylinder processes of the cells of the ganglionic layer, but a few of them are centrifugal and ramify in the inner plexiform and inner nuclear layers, where they end in enlarged extremities. Optical coherence tomograghy (OCT) is an imaging technique which utilizes the concepts of interferometry. RNFL is measured in the peripapillary region with circular scan of 3.4 mm diameter on the optic nerve head. Measurements of RNFL thickness are shown in a TSNIT orientation and are compared to age matched controlled individuals. Cross sectional observational study will include 150 eyes divided into two groupsaccordging to the age. First group will include 75 eyes of normal subjects in the second decade of liferanging from 20 to 29 years old with mean 24.73± 3.31 years old, other group will include 75 eyes of normal subjects in the fifth decade of liferanging from 50 to 59 years old with mean 52.83 ± 2.74.They are 40 males and 35 females where the male: female ratio was 1.14:1 while group B contains 23 males and 52 females where the male: female ratio was 0.44:1.The spherical refraction of group A ranges from – 7.5 to 0.75 diopters, with mean -1.19±1.85 diopters. While that of group B ranges from – 5.5 to 3.25 diopters, with mean -4.1±1.86 diopters. All patients will be subjected to full ophthalmological assessment, OCT will be done to all subjects to evaluate the RNFL thickness in the peripapillary area. 150 eyes with 63 males and 58 females. The mean of their age (52.832.74), with. Mean of their refraction (-1.191.85. As regard Superior quadrant RNFL thickness, there was highly statistically significant difference between both groups, with p-value <0.001 HS. As regards the inferior quadrant RNFL thickness, there was no statistically significant difference between both groups, with p-value 0.310 NS. There is age related decrease in Retinal nerve fiber layer thickness, with significant age related reduction in the superior peripapillary quadrant and with no significant age related variation in inferior quadrant.