Background: Hyperandrogenism is characterized by excess in production of androgens by the ovaries and/or adrenal glands. The most common clinical manifestations of hyperandrogenism in women are hirsutism, androgenic alopecia, acne vulgaris and seborrhea. Recently prostate specific antigen (PSA) was found in a wide variety of female tissues and fluids such as the breast, ovary, milk and amniotic fluid. Increased serum PSA level in a significant number of women with hirsutism, anticipates a significant direct correlation with serum androstanediol glucuronide. Objective: To estimate the possible use of prostate specific antigen as a reliable diagnostic marker of female hyperandrogenism. Method: Thirty premenopausal female patients with hyperandrogenism and 30 age- matched healthy control females were detected for the levels of serum PSA and free testosterone. The results were statistically analyzed. Results: The level of serum PSA was found to be significantly higher in the hyperandrogenism patients than in the healthy controls (P < 0.001), and it showed a weak positive correlation with free testosterone (r = 0.40, P < 0.037). Conclusion: The increased level of PSA could be used as a diagnostic marker of hyperandrogenism in females.