Objectives: The objective of this study was to detect the seroprevalence of anti-HAV antibodies in the middle socio-economic class (SES), medical students, Kasr El Aini hospital, Cairo University, Egypt. Methods: BioELISA test was done to detect HAV antibodies, using sera of 182 volunteered medical students (preclinical, clinical) and house officers of the middle SES, aging 17-30 years old. Results: The serosurvey revealed that 19.2% of the studied subjects were seronegative; with the age 17-20 years old and the preclinical group of students were the most prone to infection with HAV. Analysis of data obtained from questionnaire about the risks of exposure to the HAV infection revealed significant statistical difference as regards the frequency and the level of place of outdoor food intake and the history of food borne diseases; while no significant statistical difference was detected as regards: Sex, SES (father’s occupation, residence), sources of drinking water, exposure to jaundiced patients, hospital admission, traveling and camping. The multivariate regression analysis revealed increased seroprevalence with the low level of outdoor food intake at the different education grading in the absence and presence of food borne diseases. The stepwise logistic regression analysis revealed increase in the seroprevalence of HAV antibodies with increase in the age and the low level of outdoor food intake.Conclusions: There is a shift of seroprevalence with age from childhood into adulthood. The seroprevalence of HAV antibodies increases with low level of outdoor food intake. Is there a need for vaccination the young generation, especially the medical students prior to their clinical practice.