This study is an observational study aimed to evaluate children dental anxiety during treatment.Subjects and methods: The dental anxiety degree of 200 children, 100 males & 100 females with age range of 3-6 years old (mean age=4.8) who had primary tooth indicated for extraction was assessed using Venham Picture Test. In this study the children neither had any previous visits to a dentist nor did they have any history of any systemic disease nor hospitalization. Children were selected from outpatient’s clinics of Pediatric Dentistry and dental public health department - Faculty of Oral and Dental Medicine - Cairo University.Result: The study comprised of 100 males (mean age=4.88, SD=0.13) and 100 females (mean age=4.85, SD=0.12). Statistical analysis revealed no significant difference in age between males and females (p-value=0.233). In the present study Venham Picture Test was used to assess children’s dental anxiety at different times during dental treatment. An insignificant difference between males and females for all questions was noted.Conclusion: No significant difference in age between males and females was found. Gender has no impact on the dental anxiety. Regarding children, the highest anxiety provoking situations were taking anesthesia injection. While the lowest anxiety provoking situations were at waiting room.