This research experimentally and analytically investigated the flexural behavior of an innovative cold formed Bi-steel-concrete composite beam cast with concrete slab. Three beams were fabricated by different composite techniques (means) between the composite web and the concrete slab and were tested under static loading. All beams were designed to ensure flexural-dominant failure mode. Test results showed that the shear studs used with staggered configuration prevented the steel-concrete interface slip and controlled the local buckling of the steel plate. The use of steel transverse bars as anchor bars which connect the web plate with the concrete slab ensured preferable structural properties compared to the other techniques. In addition, the results were compared to analytical model proposed by the ACI code. A comprehensive discussion based on the effect of the proposed interlocking techniques on the yielding load, local buckling load, maximum strength, load-deflection, ductility, and the final failure mode is presented.