Introduction: Some harmful compounds could be dangerous if used in the medical field, Physical, chemical, and green synthesis processes produce metal oxide nanoparticles, this research aims to study extracting Zinc Oxide nanoparticles in a green way using orange fruit peel, to reduce using toxic chemicals in nanoparticle invention and enhance the antibacterial activity, biological and medical applications of Zinc Oxide/Graphene Oxide nanoparticles. Methods: The study utilized the electrospinning method to fabricate the Polyurethane nanofibers, and a green synthesis approach was employed to produce zinc oxide. Structural properties of Zinc Oxide/Graphene Oxide composite nanomaterials were characterized with X-ray diffraction (XRD), Fourier-Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and Transmission Electron microscope (TEM). Results: the results of the study indicate that ZnO can be synthesized in a green way from orange peel and ZnO/GO-embedded polyurethane nanofibers exhibited strong antibacterial activity toward Escherichia coli (E. coli). As evidenced by the observed SEM and TEM analysis, FTIR spectroscopy, and XRD characterization. Conclusion: The research findings suggest that using polyurethane nanofibers via electrospinning could be an applicable curriculum for addressing the challenges associated with antibacterial activity and eco-friendly compounds in the medical field.