Recently, there has been a great deal of interest among researchers in the production of lactic acid and its conversion to polylactic acid. Environmental issues such as global warming and plastic pollution are forcing society to look for alternatives to traditional plastics. Polylactic acid (PLA), made from lactic acid (LA) and one of the most famous environmentally friendly biodegradable bio-based polyesters, has been extensively studied for its applications and is a promising candidate for petroleum-based plastics. It is considered a good alternative. The goal of this research was to produce lactic acid from agricultural wastes as a low-cost, renewable substrate and to produce the most commercially successful bioplastic from agricultural wastes. Eighteen bacterial strains were isolated from agricultural wastes (cotton waste, banana waste, potato waste, and Nile Flower). Chemical hydrolysis of agricultural wastes is carried out using hydrochloric acid, sulfuric acid, and sodium hydroxide. Cotton and banana waste produced the highest concentrations of total reducing sugars of 6568.99 and 7460.37mg/L respectively, through chemical hydrolysis. Biological hydrolysis of these wastes was also achieved. Optimal conditions for lactic acid production have been promoted as temperature of 30◦C, pH 7, inoculum size of 10% (v/v), and agitation rate at 150 rpm under the stationary growth incubation of 24 h. The most promising bacterial isolate for lactic acid production was identified by 16S rRNA as Enterococcus Gallinarum with similarity of 99%. The isolate Enterococcus Gallinarum, recommended for using in lactic acid production, showed the highest lactic acid concentrations of 4.516 g/L after 24 h, as qualitatively determined by high performance liquid chromatogram (HPLC) analysis.