Port-A catheters are commonly used for chemotherapy administration, and proper care is crucial to prevent complications such as infections, thrombosis, or catheter malfunctions. Evaluating nurses' understanding of the correct procedures for maintenance, cleaning, and monitoring of the catheter. Aim: To assess the level of nurses' knowledge and practices in caring for implanted Port-A-catheter care for chemotherapy patients Design: A descriptive research design was utilized. Setting: Medical Oncology and Malignant Hematology Department at South Egypt Cancer Institute. Sample: The researchers selected 30 nurses as part of a convenience sampling method. Tools: A structured interview questionnaire for nurses : observational checklists for nurses caring for patients with port-A-catheter Results: The majority of the nurses in the study displayed unsatisfactory knowledge and practices regarding Port-A-Cath care for chemotherapy patients. Overall, the mean ± SD percent score for nurses' knowledge was 51.20 ± 1.95. The majority of nurses demonstrated an inadequate level of practice, with an overall mean ± SD percent score of 68.23 ± 7.699. The only significant correlation observed was between total practice and educational level, which had a strong positive correlation (r = 0.562, p = 0.001). Conclusion: The research findings showed that more than half of the nurses in the study had an unsatisfactory level of knowledge regarding Port-A-catheter care. Additionally, the majority of the nurses exhibited an inadequate level of practice. Recommendations: Regular educational sessions on port-a-catheter care should be held for nurses involved in the care of chemotherapy patients.