Background: Peripheral venous catheters, which are often used at the critical care units for newborns, may lead to serious complications; the most frequent issues associated with Catheters for peripheral veins implantation are infiltration and Phlebitis. Aim: This study's aim was to evaluate the effect of the educational program about Management of Intravenous Infiltration and Phlebitis on Nurses' Performance at the critical care units for newborns. Subjects and Method: Design: a quasi-experimental research design was used. Setting: The research carried out in the Universal Health Insurance Hospitals (Al-Nasr Specialized Hospital, Al Salam, Obstetrics and Gynecology hospital, and Alhayat hospital) in Port Said City. Subjects: A convenient sample of all nurses who are currently employed at the neonatal intensive care units at the time of the study. Tools: Three instruments for gathering data consisted of; Self-administered Questionnaire for nurses' knowledge regarding Management of Intravenous Infiltration and Phlebitis, observational checklist for Management of Intravenous Infiltration and Phlebitis, Pediatric peripherally inserted IV (PIV) Rating Scale. The Results: The results indicated that there is a statistically significant improvement of knowledge and practice of IV infiltration and phlebitis after program, this is showed in decreasing IV complication after program compared with preprogram. Conclusion: the educational program about Management of Intravenous Infiltration and Phlebitis had positive effect on the skills and expertise of nurses in relation to phlebitis and infiltration management, and prevention. The instructional program worked well for neonates on decreasing IV complication. Recommendations: Continuous education should be provided to nurses in order to instruct them in managing and preventing PIV complications.