Background: Cardiotocography has been considered the primary tool for monitoring fetal health during antepartum and intra-partum periods. Cardiotocography detects any changes of the fetal heart rate tracings that might jeopardizes the fetal condition or posing risk of death or distress. The aim of the study was to evaluate effect of educational program on nurses' performance regarding cardiotocography at labor units. Subjects and method: A quasi-experimental research design was used to conduct this study. Setting: The study was carried out at labor units of obstetrics and gynecological department of Tanta Main University Hospital, El- Menshawy General Hospital and El- Mabra Hospital. Sample: The study comprised all 54 nurses who were employed in the aforementioned settings. Tool (I): Nurses' knowledge regarding cardiotocography during labor. It included Part (1): Socio-demographic characteristics of nurses and Part (2): Nurses' knowledge regarding cardiotocography during labor. Tool (II): Nurses' Practices observational checklist regarding cardiotocography. Results: High level of nurses' knowledge and satisfactory practices regarding cardiotocography were reported immediately and three months after program implementation in contrast to low and unsatisfactory practices before educational program implementation. Conclusion and Recommendations: The cardiotocography educational program had a positive effect on improving the level of knowledge and practices among the studied nurses working at labor units. Regular annual training courses and workshops should be conducted for maternity nurses regarding cardiotocography monitoring and its traces.