Bold Basal Media was used to culture the unicellular microalga Cosmarium sp. under carefully monitored conditions. In order to aid in microalga culture and extract useful compounds, we looked into biochemical characterizations applying nonmetals. Although the vast majority is cognizant of how harmful metal nanoparticles may be for microalgae, nanotechnology has a significant effect on a lot of other living organisms. The aim of this research is to evaluate the effects of zinc oxides and zinc oxide nannoparticles on Cosmarium sp. growth and induce resistance in order to improve the manufacture of high-value microalgae products. Zinc oxides and zinc oxide nannoparticles were added during its growth, with concentrations ranging from 0 to 300 mg/l. Studies comparing the effects of zinc oxide nanoparticles have shown that the treated cells accumulated more lipids and biomass than the non-exposed cells. The polymer's chloroform extract for Cosmarium sp. exposed to nannoparticles yielded eighteen distinct biodegradable chemicals, according to the results of GC/ MS analysis. Acetic acid, 2-methylpropyl ester (isobutyl acetate), Heptanol acid, nonanoic acid, and Heptanol acid were the main compounds found. Isobutyl acetyl citrate had the highest molecular weight of 402 and was the most prevalent compound with a total percentage of 29 and molecular weight of 116.08. Cosmarium sp. is capable of producing polyhydroxyalkanoate, which is about to be used extensively as a bioplastic, at different levels from zinc oxide nanoparticles.