Abstract Background: Personality traits and technology may affect how well nursing utilize their abilities and strategies to achieve their objectives and innovations at the place of work. So, nurses' personalities have a great influence on their creativity and innovative behavior involved using artificial intelligence. The study aimed to assess the nurses' personality trait as a mediator between their artificial intelligence perception and innovative work behaviors. Research design: A descriptive correlational research design was applied. Setting: The research was conducted on different hospitals in Minia City. Sample: A Purposive sample with a total number of 329 nurses. Tools of data collection: Three tools were used, 1st tool was Big Five Inventory, 2nd tool was Perception of Artificial Intelligence Scale and the 3rd tool was Innovative Work Behaviors scale. Results: Revealed that the highest mean scores of nurses were for conscientiousness traits, followed by openness and agreeableness personality. Moreover, fewer than fifty percent of nurses had a high as well as a moderate level of perception of artificial intelligence. Also, approximately three quarters of nurses had high level of innovative work behaviors. Conclusion: There was a significant positive correlation between nurses' total perception of artificial intelligence as well as their innovative work behaviors and all dimensions of personality traits except neuroticism dimension. Recommendations: Training programs should emphasize the benefits and practical applications of AI in nursing to alleviate fears and resistance, particularly among those with high neuroticism personality.