Background: The application of artificial intelligence (AI) in healthcare may help address some of the issues that global healthcare systems are now facing opportunities AI was a bridge between healthcare and technology in nursing today. Aim of the study: to determine nurses' opportunities and challenges regarding application of artificial intelligence in intensive care unit. Research design: A descriptive research design was used in this study. Setting: This study was conducted at intensive care units and medical emergency unit at Al-Eman general Hospital. Sample: Was included a convenience sampling of available critical care nurses work at the previous mention setting. Tools: Tool one: Nurses' opportunities regarding application of artificial intelligence questionnaire; tool two: Nurses challenges regarding application of artificial intelligence questionnaire. Results: about 58 % from nurses had good knowledge regarding AI with mean &SD had 35.24± 11.07. Also, 69 % from nurses had satisfactory nurse's challenges regarding AI. The current study revealed relation between total score of nurse`s challenges regarding AI and total score of nurse`s knowledge with no relation between the both total score levels. Conclusion: The most of critical care nurses had good knowledge regarding opportunities of AI. Additionally, nurses had satisfactory nurses' challenges regarding AI. Recommendations: Performing scientific research concentrated on AI at ICU at Egypt and preparing training program for nurses about opportunities and AI challenges facing them.