Background: An essential tool commonly used in neonatal intensive care units for critically ill neonates in need of ongoing monitoring and resuscitation treatments is a peripherally inserted central catheter. One active learning strategy that enhances learning, increases cognitive function and improves memory recall is the mind map. Aim: To evaluate the impact of mind mapping on pediatric nurses' performance regarding peripherally inserted central catheters at neonatal intensive care units.  Design:  To achieve this study's goal, a quasi-experimental research approach was employed.  Setting: The study was conducted in neonatal intensive care units, at Sohag University Hospitals. Subjects: The study included all available nurses (50) working in Neonatal intensive care units in the above-mentioned study setting. Tools: Three tools were used for data collection: Tool (I): A structured interviewing questionnaire which consisted of two parts; part (1)  demographic characteristics of the nurses; and part (2) nurses' opinion regarding the utilization of the mind mapping method in training. Tool (II): Nurses' knowledge about peripherally inserted central catheters and mind mapping method (pre/post), Tool (III): Observational checklist (pre/post). Results: There was a highly statistically significant difference between total knowledge of mind mapping and pediatric nurses' performance regarding peripherally inserted central catheters. Conclusion: The present investigation found that the mind mapping technique improved the performance of pediatric nurses with peripherally inserted central catheters in neonatal intensive care units. Recommendations: According to the study, for pediatric nurse education and training, mind mapping ought to be employed as a teaching method.