Background: Medical waste management is an important aspect of healthcare delivery, Proper waste management practices are essential to prevent the spread of infection, reduce environmental pollution, and safeguard public health. Study aim: to assess healthcare worker's performance based on occupational safety regarding waste management. Subjects and methods: this cross-sectional descriptive study was carried out at the main primary health care centers in Fayoum city and the governmental hospitals in the city on 200 healthcare workers. Data were collected using two different versions of self-administered questionnaires and observation checklists, for nurses .Results: Nurses' age ranged between 22 and 59 years. 33.3% of the nurses had satisfactory total knowledge of waste management. And 68.8% of the nurses had a positive total attitude. While 61.5% of the nurses reported having adequate practice, only 34.4% of them had adequate observed practice.Conclusion and Recommendations: the nurses have deficient knowledge of waste management, with more positive attitudes and less adequate practice. Recommended sufficient resources should be allocated, regarding safe waste management.