Communication is a core element in every phase of daily events of staff nurses. Having good communication skills increase team cohesion through allowing staff nurses to adhere to every one more. Highly cohesive teams promote cooperation, as nurses work together in the direction of a shared goal, exchange information, and leveraging every one capabilities as well as strengths. Aim: The present study aimed to investigate the effect of communication skills training program on staff nurses' team cohesion at Mansoura University Children's Hospital. Methods: Quasi-experimental design was used. This study was carried out in wholly medical as well as surgical departments at Mansoura University Children's Hospital. The whole sample was (n=115) staff nurse (convenience sample). Data was collected through using three tools; Communication Skills Knowledge Questionnaire, Communication Skills Questionnaire and The Erlangen Team Cohesion at Work Scale. Results: The main results of the existing study showed that the staff nurses' owned higher communication knowledge as well as skills during post in addition to follow up program and higher team cohesion. There was a positive correlation amongst staff nurses' communication knowledge, skills as well as team cohesion. Recommendations: Evaluate staff nurses` knowledge periodically to identify the points that require training in order to enhance their communication skills as well as team cohesion. Implement a continuous education as well as training programs for encouraging in addition to increasing staff nurses' knowledge plus skills around the communication skills.