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413975

Combating Biofilm: Melatonin's Role in Mitigating Multidrug-Resistant Uropathogens in Cancer

Article

Last updated: 25 Feb 2025

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Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Multidrug-resistant (MDR) bacterial infections, particularly urinary tract infections (UTIs), are prevalent in cancer patients, due to long-term immunosuppression. Uropathogenic bacteria like Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa, complicate treatment through biofilm formation, enhancing antibiotic resistance. Recent studies has explored the potential of melatonin, an antioxidant hormone, to disrupt bacterial biofilms. Objective(s): This study analyzes the antibiotic resistance of uropathogenic bacteria from cancer patients and evaluates the efficacy of melatonin in inhibiting biofilm formation in these MDR strains. Methodology: 65 urine samples from cancer patients were collected at Sabha Oncology Center. Bacteria were identified using standard techniques, and antibiotic susceptibility was evaluated via the Kirby-Bauer method. Biofilm formation was measured using crystal violet staining, and melatonin's antibiofilm activity was tested from 10 µg/mL to 1000 µg/mL. Results: E. coli was the most prevalent pathogen (32.69%), followed by K. pneumoniae (17.30%) and P. aeruginosa (15.38%). All isolates exhibited high resistance to amoxicillin/clavulanic acid and cefotaxime with Strong biofilm. Melatonin notably inhibited E. coli biofilm formation at higher concentrations, with lesser effects on K. pneumoniae and P. aeruginosa. Conclusion: The prevalence of MDR uropathogens and their biofilms formation underscore the need for new therapies in cancer patients. Melatonin may serves as a complementary treatment to inhibit biofilm, particularly in E. coli. Additional research is required to assess its clinical application in managing biofilm-associated infections.

DOI

10.21608/ejmm.2025.327626.1358

Keywords

multidrug-resistant bacteria, Biofilms, antibiofilm activity, Urinary tract infections (UTIs), Cancer patient infections

Authors

First Name

Shamsi

Last Name

Shamsi

MiddleName

S.

Affiliation

Sebha University, Faculty of Medical Technology, Department of Medical Laboratory, Sebha, Libya

Email

sha.saad@sebhau.edu.ly

City

sebha

Orcid

-

First Name

Khajida

Last Name

Ahmed

MiddleName

M.

Affiliation

Sebha University, Faculty of Medicine, Department of Microbiology, Sebha, Libya

Email

jamelaalshaye@gmail.com

City

sebha

Orcid

-

First Name

Huda

Last Name

Al-Griw

MiddleName

H.

Affiliation

University of Tripoli, Faculty of Veterinary Medicine , Department of Microbiology and Parasitology, Libya

Email

jami.alsayah@fsc.sebhau.edu.ly

City

-

Orcid

-

First Name

Abdelgani

Last Name

Abobaker

MiddleName

M.

Affiliation

Sebha University, Faculty of Science, Department of Botany, Sebha, Libya

Email

ab.radu@sebhau.edu.ly

City

-

Orcid

-

First Name

Fatima

Last Name

Abdulnabi

MiddleName

A.

Affiliation

Sebha University, Faculty of Science, Department of Botany, Sebha, Libya

Email

fa.abdulsalam@fsc.sebhau.edu.ly

City

sebha

Orcid

-

First Name

Mofida

Last Name

Alhashemi

MiddleName

S.

Affiliation

Sebha University, Faculty of Science, Department of Microbiology, Sebha, Libya

Email

-

City

-

Orcid

-

First Name

Heba

Last Name

Alshebani

MiddleName

O.

Affiliation

Sebha University, Faculty of Science, Department of Microbiology, Sebha, Libya

Email

-

City

-

Orcid

-

First Name

Nabeha

Last Name

Hassan

MiddleName

F.

Affiliation

Sebha University, Faculty of Science, Department of Microbiology, Sebha, Libya

Email

-

City

-

Orcid

-

Volume

34

Article Issue

3

Related Issue

53640

Issue Date

2025-07-01

Receive Date

2025-02-15

Publish Date

2025-07-01

Print ISSN

1110-2179

Online ISSN

2537-0979

Link

https://ejmm.journals.ekb.eg/article_413975.html

Detail API

http://journals.ekb.eg?_action=service&article_code=413975

Order

413,975

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Combating Biofilm: Melatonin's Role in Mitigating Multidrug-Resistant Uropathogens in Cancer

Details

Type

Article

Created At

25 Feb 2025