Abstract: Background: Poisoning is one of the most common causes of admission to pediatric emergency and intensive care units. Pediatric poisonings are a common and preventable cause of morbidity and mortality throughout the world. Therefore, there is an intense need to educate mothers on how to provide first-aid management when their children are exposed to poisons. The purpose of this study was to assess   the effect of instructional module on mothers' knowledge and practice regarding management of household poisoning among their children. Design: A quasi-experimental design was used (pre& posttest). Setting: The study was conducted at El- Qebly Maternal and Child Health Center (MCH) at Shebin El-Kom city. Sample: A purposive sample of 70 mothers was selected with their children to carry out this study. Instruments: Two instruments were used in this study, Structured Interviewing Questionnaire (Characteristics of studied sample, Mother's Knowledge about household poisoning) and Mother's Reported Practice Checklist. Results: The results of this study showed that there were an improvement on mother's knowledge about household poisoning on post intervention than pre intervention (1.86 ± 0.18 VS 0.71 ± 0.26). Also, all mothers had a satisfactory level regarding the preventive measures on posttest compared to pretest (100% VS 50% respectively), In addition, All mothers had a satisfactory level regarding the preventive measures on posttest compared to pretest (100% VS 67.1% respectively) regarding first aid management post intervention. Conclusion: The study concluded that instructional Module was effective in improving knowledge and appropriate practice related to household poisoning management in children. So, it was recommended that establishing preventive educational programs for mothers regarding safety home environment and household poisoning management of their children is important.