Background: Surgical site infections (SSIs )also called which develop within 30 to 90 days after surgery or as a result of surgical intervention at the incision site, deep tissues, organs, or cavities .Aim: This study aimed to investigate the nursing knowledge and practices regarding bundle of care for prevention of surgical site infection. Research design: A descriptive research design was utilized for conducting the study. Setting: The current study was conducted at the General Surgical Unit at Beni-Suef University Hospital. Subjects: .A Convenience sample (40) of all available staff nurses (males & females) used in current study. Tools for Data Collection: Two tools were used: tool (1) Nurses self administered questionnaire, and tool (2) Nurses' observation checklist. Result: the study revealed that (55%) of studied nurses were aged between 20 and 25 years with mean age (25.45±1.67), (65%) of them were female.. (52.5%) studied of them had technical nursing institute,. ( 65% )of studied nurses have unsatisfactory level of knowledge regarding bundle of care for prevention of surgical site infection and (90%) of them have incompetent level of practice regarding surgical site infection bundle of care. Conclusion: Less than two third of the studied nurses had unsatisfactory total level of knowledge regarding bundle of care for prevention of surgical site infection and the majority of them had incompetent practice level . Recommendations Developing a simplified and comprehensive booklet .Unit protocols regarding hospital acquired infections especially SSI .Routinely updating knowledge and practice of nurses through in-service continuing educationprograms.