Background: A vital component of both physical and mental wellness is oral health; Early Childhood Caries (ECC) affects children globally, particularly in Egypt, affecting school attendance, educational achievements and work productivity. Early action is essential for preventing. This study aimed to analyze how an educational program has affected mothers' knowledge and practices around the oral dental health of their young children. Design: In this work, a quasi-experimental research design was employed. Setting: The current investigation was carried out at Sohag University Hospital's outpatient dental clinic. Subjects: A convenient sample of 100 mothers attended with their young children. Tools: Two tools used in this study, Tool (1): An organized questionnaire sheet for an interview includes part (1): Socio-demographic traits of the young children's mothers, part (2): sociodemographic traits of the child and part (3): Mothers' knowledge of the dental health of their children, Tool (II): Assessment sheet for reported practices. Results The majority of mothers (94%) possessed a high level of experience in post-program implementation. Furthermore, most of the mothers in the research (88%) showed competence in implementing the program after it ended. Conclusion: The instructional program increased mothers' knowledge as regard to their young children's dental health. There was a positive correlation between mothers' general knowledge and practices prior to and during the program's implementation. Recommendation: must be holding frequent workshops and training sessions for mothers about the dental health of their young children