Study of Gardenia jasminoids 'Varigata' took place over the course of two years (2021–2023) in the Tissue Culture Laboratory -Horticulture Research Institute, Agricultural Research Center, Giza, Egypt. This study sought the micropropagation protocol for this promising cultivar of Gardenia used as an indoor plant and recently imported. The aim of this study is to achieve the best result for the various stages of propagation (establishment, multiplication under the influence of cytokinins and fluorescent lights, rooting and acclimatization stages). Since it is imported cultivar. Shoot tip explants sterilized with Mercury chloride (MC) at 0.1% for 10 min produced the best free-contaminated and survival %. Establishing explants on full Murashige and Skoog (MS) basal medium supplemented with 2.0 mg/l BAP produced the largest shootlet number/explant, while ½ MS salt strength free hormone (control) recorded the longest shootlet (cm). For florescent lights, a white florescent lamp recorded the highest shootlet number/explant and leaf number/shootlet, while the shootlet length increased under a yellow florescent lamp. For rooting, culture shootlet on ½ MS salt strength recorded the longest plantlet, roots (cm), and largest plantlet, leaf, and root number/shootlet. Acclimatization on peat moss recorded very good survival and growth.