Acute myocardial infarction (AMI) is one of the most common causes of morbidity and mortality across the world. AMI is associated with an intelligible imbalance in some trace elements such as zinc (Zn), copper (Cu), manganese (Mn), iron (Fe), and selenium (Se), which are significant for immune and cardiovascular function. This study aimed to evaluate the clinical significance and correlation of serum trace elements and electrolytes in Iraqi patients as adjunctive parameters for a better prognosis of AMI. This study contained 120 subjects who were divided evenly into patients and control groups for the period between September and December 2021. Serum trace elements were measured using atomic absorption spectroscopy while the Jokoh analyzer (EX-D/EX-DS, Tokyo, Japan) was used for serum electrolytes. There was no significant difference in BMI or age between patient and control groups (p>0.05) and these findings provide an excellent opportunity to conduct a case study comparing patients with acute myocardial infarction to healthy controls. The serum Zn, Se, Fe, and Na+ of AMI patients were significantly lower when compared with that of the control group (p < 0.001) and significantly higher for Cu while, non-significant for Mn, K+, and Cl− (p>0.05).