308641

Anti-HBV Efficacy of <i>Ficus</i> Plant's Leaf Extract against the Most Dominant HBV Genotypes in the Nile Delta Region of Egypt

Article

Last updated: 01 Jan 2025

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Abstract

Hepatitis B virus (HBV) infects millions of people worldwide annually, causing chronic liver diseases such as cirrhosis, fibrosis, and hepatocellular carcinoma. HBV has nine known genotypes (A, B, C, D, E, F, G, H, and I) and a tentative tenth genotype (J). Identifying HBV genotyping is crucial since different genotypes and sub-genotypes can influence treatment outcomes and responses to antiviral therapy. Therefore, we employed conventional polymerase chain reaction (PCR) to determine the HBV genotypes in three Egyptian governorates: Dakahlia (DK), Gharbia (GHR), and El-Sharkia (SHR). Specific primers for various HBV genotypes (A, B, C, D, E, and F) were used for this purpose. Out of 38 samples, three samples (7.8%) were found to have the D genotype, and four samples (10.5%) were identified as genotype C. The genotypes that were discovered underwent deep sequencing, which revealed two bands in the sequence results that were 99% identical to genotype D. The remaining bands could not be sequenced or gave unclear and incorrect sequence data. We evaluated the leaf extracts of three Ficus species (F. mysorensis, F. deltoidei, and sycomorus) as antiviral substances against HBV infection using an MTT assay. The results showed promising antiviral effects of these extracts, which could potentially aid in stopping the progression of HBV infection. Our findings indicate that conventional PCR with deep sequencing can accurately identify HBV genotypes, and Ficus plants may serve as an excellent supplement to standard treatment for HBV infections.

DOI

10.21608/ejbo.2023.183525.2219

Keywords

Anti-HBV, conventional PCR, <i>Ficus mysorensis, Ficus deltoidei, Ficus sycomorus</i>, Natural plant extract

Authors

First Name

Mohammed A.

Last Name

Eid

MiddleName

-

Affiliation

Botany and Microbiology Department, Faculty of Science, Tanta University, Tanta Egypt

Email

mohamed.eid@science.tanta.edu.eg

City

Tanta

Orcid

0000-0003-4547-4675

First Name

Mohamed S.

Last Name

Youssef

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt

Email

dr_gadelkarim@yahoo.com

City

-

Orcid

-

First Name

Yehia A.

Last Name

Mahmoud

MiddleName

-

Affiliation

Botany and Microbiology Department, Faculty of Science, Tanta University, Tanta Egypt

Email

yehia.mahmoud@science.tanta.edu.eg

City

-

Orcid

-

First Name

Reham

Last Name

Sedeek

MiddleName

-

Affiliation

Medical Analysis Fellow, Urology and Nephrology Center, Mansoura University, Mansoura, Egypt

Email

sreham444@yahoo.com

City

mansoura

Orcid

-

First Name

Hazem H.

Last Name

Saleh

MiddleName

-

Affiliation

Medical Analysis Fellow, Urology and Nephrology Center, Mansoura University, Mansoura, Egypt

Email

hazemhamed@mans.edu.eg

City

mansoura

Orcid

0000-0003-1890-2005

First Name

Gamal K.

Last Name

Kasem

MiddleName

-

Affiliation

Tropical Medicine and Fever Department, Faulty of Medicine, Tanta University, Tanta, Egypt

Email

gamal.kassem@med.tanta.edu.eg

City

tanta

Orcid

-

First Name

Asaad

Last Name

Gaber

MiddleName

-

Affiliation

Medical Analysis Fellow, Urology and Nephrology Center, Mansoura University, Mansoura, Egypt

Email

asaadgaber@yahoo.com

City

-

Orcid

-

Volume

63

Article Issue

3

Related Issue

43286

Issue Date

2023-09-01

Receive Date

2022-12-26

Publish Date

2023-09-01

Page Start

1,069

Page End

1,082

Print ISSN

0375-9237

Online ISSN

2357-0350

Link

https://ejbo.journals.ekb.eg/article_308641.html

Detail API

https://ejbo.journals.ekb.eg/service?article_code=308641

Order

24

Type

Regular issue (Original Article)

Type Code

111

Publication Type

Journal

Publication Title

Egyptian Journal of Botany

Publication Link

https://ejbo.journals.ekb.eg/

MainTitle

Anti-HBV Efficacy of <i>Ficus</i> Plant's Leaf Extract against the Most Dominant HBV Genotypes in the Nile Delta Region of Egypt

Details

Type

Article

Created At

30 Dec 2024