Background: Portfolio is a measurement tool that can document student learning, performance, and achievement of the key objectives. Aim: to assess the effect of educational program about internship portfolio on intern's knowledge and satisfaction. Subjects and methods: Pre-experimental one group pretest posttest design was used to conduct the present study. Setting: This study was conducted at Suez Canal University hospitals and private hospitals such as (International Medical Center, Ganzouri Hospital and 57357 Hospital) Sample: Academic nursing experts (N= 10) and all available nursing intern's during data collection period (N= 100). Tools of data collection: Data was collected using four tools through the study phases. The designed portfolio; Opinionnaire regarding validation of the designed portfolio; Nursing interns' knowledge questionnaire about portfolio and nursing interns' satisfaction questionnaire. Results: There was a statistically significant difference of nursing interns' knowledge regarding portfolio between before and after training program. There was a statistically significant difference of total mean score of nursing interns' satisfaction regarding portfolio before and after portfolio implementation. Conclusion: There is an improvement in nursing interns' knowledge regarding developed portfolio after training program. The nursing interns have high satisfaction levels about developed portfolio. It Recommended to: Adopting the developed portfolio for the nursing interns at Faculty of Nursing – Suez Canal University was recommended beside to conducting regular training program for nursing interns on portfolio and how to use it. it.