Hemodialysis (HD) is the most common dialysis modality which is used for sustaining life of patients with end stage renal disease. However, it is linked to various complications and places unique physiological and psychological stress on HD patients. Therefore, comprehensive and competent nursing care is required to meet complex health needs of HD patients and improve health outcomes. Objective: Assess nurses' practices regarding care of patients undergoing hemodialysis. Setting: The study was carried out at two hemodialysis units of EL-Mowasat University Hospital and the Medical Research Institute Hospital in Alexandria. Subjects: A convenience sample of 50 nurses providing direct care to hemodialysis patients was selected. Tools: A single tool was employed for gathering data: Observational checklist regarding nursing care for HD patients. Results: Illustrated that 54.0% of nurses had poor overall practices related to the care of HD patients and 38.0% of them had fair practices. While, 8.0% of nurses had good practices. Conclusions: Most HD nurses had inadequate practices regarding care of HD patients during all phases of HD procedure and poor practices and adherence level with infection control measures at HD units. Recommendation: Continuous assessment of the nurses' practices and performance at HD units is essential to ensure that they comply with HD guidelines, identify and modify any defects in the provided care to improve the quality of nursing care.