Introduction: Evidence-based practices guidelines for women undergoing hysterectomy are considered a strategy for promoting their health and wellbeing. Self-care is the ability of individuals, families, and communities to promote health, prevent disease, maintain health, and to cope with illness and disability with or without the support of a healthcare provider. Aim: This study aimed to evaluate the effect of implementing evidence-based practices guidelines on women's knowledge and practices regarding self-care after hysterectomy. Subjects and Methods: A Quasi-experimental (one group pre & post-test) design was utilized in this study. A purposive sample of 60 women was recruited in this study that was conducted at gynaecological departments of Minia university hospital, Egypt. The study utilized four tools, (I) Structured interview questionnaire, (II) Structured questionnaire on women's knowledge, (III) Women's self-reported practices, & (IV) Follow up questionnaire. Results: The study's main findings revealed that (88.30%) of women had inadequate knowledge and (98.30 %) of them had unsatisfactory practices regarding self-care after hysterectomy in the pre-test.  While, after application of evidence-based practices guidelines, it was observed that there was a highly statistically significant improvement (P ≤ 0.001) in women's total knowledge and practices. The most common health problems experienced within 6 weeks after hysterectomy was night sweats & hot flashes (86.6 %). Conclusion: Implementing evidence-based practices guidelines was effective and significantly improved women's knowledge and practices regarding self-care after hysterectomy. Recommendations: Discharge planning regimen for self-care practices after hysterectomy should be developed and implemented pre-and post-operatively to prevent complications