Beta
ManageSearchChatDiscover
328335

Inflammasome NLRP3 Expression in PBMCs and Plasma Interleukin-21 in Psoriasis

Article

Last updated: 28 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Genetics

Abstract

Background: Psoriasis is a persistent, immune-related complex condition that predominantly impacts both the skin and joints, involving multiple factors. Objectives: Our study aimed to make a comparison between the PBMCs expression levels of NLRP3 and plasma IL-21 levels in psoriatic patients with that of healthy controls and a correlation between these levels and the severity of psoriasis. Methodology: This observational cross-sectional study was performed on 40 psoriatic patients and 40 healthy individuals who served as the control group. We assessed the expression levels of NLRP3 in PBMCs using real-time reverse transcription-polymerase chain reaction (RT-PCR), while plasma levels of IL-21 were determined using the enzyme-linked immunosorbent (ELISA) technique. Results: Psoriatic patients showed significantly higher PBMCs expression levels of NLRP3 (P< 0.001), and insignificantly different plasma IL-21 levels from those in the control group. The expression levels of NLRP3 demonstrated a significant positive correlation with the severity of psoriasis (P< 0.001). In contrast, there was no significant correlation detected between IL-21 levels and the severity of psoriasis. Additionally, no significant correlation was observed between NLRP3 and IL-21 levels. Conclusions: NLRP3 could be a potential diagnostic and prognostic biomarker in psoriasis diagnosis with 92.5% sensitivity and 90% specificity and a promising therapeutic target. Additional studies are warranted to examine the exact contribution of IL-21 to the pathogenesis of psoriasis.

DOI

10.21608/ejmm.2024.328335

Keywords

inflammasome NLRP3, Plasma Interleukin-21, Psoriasis, PBMCs

Authors

First Name

Alaa

Last Name

Aboutaleb

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo 11562, Egypt

Email

alaaabotaleb4@gmail.com

City

-

Orcid

-

First Name

Essraa

Last Name

Hegazy

MiddleName

A.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo 11562, Egypt

Email

essraa.hegazy@residents.kasralainy.edu.eg

City

-

Orcid

-

First Name

Amal

Last Name

Elshimy

MiddleName

A.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo 11562, Egypt

Email

elshimy_amal@yahoo.com

City

-

Orcid

-

First Name

Marwa

Last Name

Amer

MiddleName

A.

Affiliation

Department of Dermatology, Faculty of Medicine, Cairo University, Cairo 11562, Egypt

Email

m_amer_84@cu.edu.eg

City

-

Orcid

-

First Name

Maha

Last Name

Kotb

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo 11562, Egypt

Email

kotbmaha@yahoo.com

City

-

Orcid

-

Volume

33

Article Issue

1

Related Issue

44331

Issue Date

2024-01-01

Receive Date

2023-12-01

Publish Date

2024-01-01

Page Start

91

Page End

98

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 33_Issue 1_Pages 91-98.pdf

PDF . 397.3kB

Link

https://ejmm.journals.ekb.eg/article_328335.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=328335

Order

328,335

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Inflammasome NLRP3 Expression in PBMCs and Plasma Interleukin-21 in Psoriasis

Details

Type

Article

Created At

28 Dec 2024