Background: A stroke is a medical emergency resulting from interrupted or reduced brain blood flow, leading to oxygen and nutrient deprivation in brain tissue. Aim: This study assessed nurses' awareness of stroke patient care at Ain Shams specialized hospital. Research design: A descriptive design was employed. Sample: A convenient sample of 100 nurses working at Ain Shams specialized hospital was included. Setting: The study took place at Ain Shams specialized hospital in various clinics: internal neurology, physiotherapy, speech, and emergency. Data collection tool: An interview questionnaire with four parts was used: 1) Socio-demographic characteristics, 2) Nurses' stroke disease knowledge, 3) Nurses' reported practice in stroke patient care, and 4) Nurses' attitude scale towards stroke patient care. Results: 42% of nurses had poor knowledge, 33% had unsatisfactory reported practice, and 67% had satisfactory reported practice. 22% displayed a negative attitude, while 78% had a positive attitude. Conclusion: Less than half of the nurses demonstrated poor knowledge, and most had unsatisfactory practice in stroke patient care. Additionally, less than a quarter had a negative attitude. A correlation was found between nurses' knowledge, reported practices, and attitude towards stroke patient care. Statistically significant relationships were observed between nurses' sociodemographic data and their knowledge, reported practices, and attitude. Recommendations: Develop a health education program and informative booklets for nurses on stroke patient care.