Background: Entrepreneurship refers to an individual's ability to turn ideas into action .So, entrepreneurial' knowledge and skills take important roles in identifying opportunities and making decisions to start entrepreneurship. Aim: Assess' nurses knowledge and skills regarding entrepreneurship. Study Design: Descriptive study design was used in this study. Setting: The study was conducted at Ain shams Medical Hospital University. Sample: A convenient sample composed of (50) staff nurses. Tools: Data was collected using nursing entrepreneurship knowledge questionnaire and evaluation of nurses' entrepreneur reported skills tool. Results: Only (20%) of staff nurses had satisfactory knowledge level regarding nursing entrepreneurship. While the majority (80%) of them had unsatisfactory knowledge level .Also, slightly less than one quarter (24%) of studied staff nurses had high level of entrepreneur reported skills, while about one third (32%) of them had moderate level and slightly less than half (44%) of them had low level. Conclusion: The majority of studied staff nurses had unsatisfactory level about nursing entrepreneurship knowledge and low level of nurses' entrepreneur skills regarding entrepreneurship. Recommendation: Create a competency-based curriculum and creative learning to support sustainable entrepreneurship learning. Review and improve curricula and content in order to incorporate an entrepreneurial culture in nursing education. Offer entrepreneurship training programs for nurses. Support the intra- entrepreneurship within the hospital.