Background: Vaginal yeast infection is a common opportunistic vaginal infection primarily caused by Candida albicans. It ranks as the second most frequent vaginal infection in women. Aim: the current study aimed to assess women's knowledge and attitude regarding vaginal yeast infection. Design: a descriptive research design was used to achieve the aim of the study. Setting: the study was conducted at outpatient clinic in Fayoum general hospital, Egypt. Sample: Purposive sampling of 100 women was utilized in the present study. Tools: three tools were used for data collection. 1) A structured interviewing questionnaire, 2) Assessment of women's level of knowledge regarding vaginal yeast infection, and 3) Women's attitude regarding vaginal yeast infection. Results: the results of the current study revealed that 96% of the studied women had unsatisfactory level of knowledge regarding vaginal yeast infection and about 77% of them had a negative attitude regarding hygiene and personal habits to prevent vaginal yeast infection. Conclusion: the study concluded that the most of women had unsatisfactory level of knowledge regarding vaginal yeast infection, and negative attitude toward regarding vaginal yeast infection. Recommendations: health education regarding vaginal yeast infection to raise awareness and improve outcomes.