Background: The There is growing worry about the effects on mothers and infants due to the increasing prevalence of cesarean sections (CS). Predicting difficulties in women with a history of cesarean section, particularly with regard to trial of labor after cesarean (TOLAC), requires a precise measurement of the thickness of the lower uterine segment (LUS). The purpose of this research is to compare the reliability of intraoperative measures with those of transvaginal and transabdominal ultrasounds in determining LUS thickness in women who have had previous CS. Methods: Included in this cohort research were 68 women, ranging in age from 18 to 40, who had a history of one prior low-transverse CS. During the 37th and 40th weeks of gestation, the TAS and TVS were measured. Elective CS was used to record intraoperative LUS thickness. The IBM SPSS Statistics software was used for the statistical analysis. The results showed that the average age of the mothers was 29.8 ± 5.9 years, and that the average gestational age was 38.4 ± 1 week. TVS recorded 4.2 ± 0.9 mm for the mean LUS thickness, while TAS recorded 6 ± 1 mm. The LUS thickness during the procedure was 3.8 ± 1 mm. The results of intraoperative measures showed a strong connection with TVS (p < 0.001). It should be noted that increased LUS thickness (5.14 ± 0.42 mm) was linked to longer inter-pregnancy intervals (≥ 18 months).