Background: Organizational culture helps nurses in the work, and self-learning resources greatly increase each nurses capacity for innovation Aim: To effect of organizational culture self-learning package in nurses' innovation work. Design: A quasi-experimental study design was used pre, immediately post self- learning package phase and follow up phase (after three months) Setting: The study was conducted at Medical departments/units at Benha University Hospital. Subject:  Consisted of45 who had at least one year of job experience  and were available during the data collection period. Tools: Include four tools I. knowledge regard Organizational culture questionnaire, II attitude regard organizational culture questionnaire, III. Performance regard organizational culture observational chick list, and IV. Innovative work questionnaire .Results:  The majority of the studied nurses (84.4%, 80 %) had an adequate level of knowledge regarding organizational culture in the immediate-post and follow up of self-learning package phases,  Additionally (84.4%, 73.3%) the studied nurses  had a positive level of attitudes regarding organizational culture in the immediate-post and follow up of self-learning package phases, also the  majority of the studied nurses (93.3%, 86.7%) had an competent performance level regarding organizational culture in the immediate-post and follow up of self-learning package phases Furthermore, (82.2%, 73.3%) of the studied nurses had a high level of innovative work implementing completing the organizational culture self- learning package in the immediate-post and follow up of self-learning package phases.Conclusion: There was a highly statistically significant positive correlation between nurses total knowledge, attitude, performance regard  organizational culture and total innovative work through study phases Recommendations: Implement organizational culture strategy through hospital strategic planning to remain competitive in today's healthcare market. Provide technology training for nurses and integrate new technological support learning media and innovation that promote organizational culture. ajority of the studied nurses (84.4%, 80 %) had an adequate level of knowledge regarding organizational culture in the immediate-post and follow up of self-learning package phases, while (84.4%, 73.3%) the studied nurses had an positive level of attitudes regarding organizational culture in the immediate-post and follow up of self-learning package phases, Conclusion: There was a highly statistically significant positive correlation about organizational culture self –learning package between total knowledge, total attitude, total performance and total innovative work in the immediate post and follow-up self- learning package phases Recommendations: Implement organizational culture strategy through hospital strategic planning to remain competitive in today's healthcare market. Provide technology training for nurses and integrate new technologist support learning media and innovation that promote organizational culture.