Background: In various nations, asthma affects 1–18% of the population. It is a common, chronic respiratory condition. Fluctuating wheezing, shortness of breath, chest tightness, and/or coughing symptoms, as well as variable expiratory airflow limitation, are the hallmarks of bronchial asthma. The aim of the study was to evaluate the association of serum NGAL with childhood bronchial asthma for diagnosis, prognosis and assessment of severity. Methods: This prospective, cross-sectional study was conducted on 40 asthmatic cases. Cases were subdivided into four asthma groups (intermittent, mild persistent, moderate persistent and severe persistent) and 20 healthy children representing the control group. Cases were subjected to history taking, Routine Laboratory investigations, special laboratory investigations as serum NGAL level. NGAL concentrations were determined with Human Neutrophil – Gelatinase - Associated Lipocalin (NGAL) ELISA Kit. Results: NGAL showed significant positive correlations with eosinophil (r = 0.676, P < 0.001), CRP (r = 0.729, P < 0.001), and asthma severity (r = 0.712, P < 0.001), while it showed significant negative correlations with FEV 1 (r = -0.723, P < 0.001) and FEV/FVC (r = -0.767, P < 0.001). No significant correlations were detected with other parameters. ROC analysis was done for NGAL to distinguish severe cases. It revealed a significant AUC of 0.945 with a 95% CI ranging from 0.841 -1 (P < 0.001). The best cut-off point was > 190, at which sensitivity and specificity were 87.5% and 100%, respectively. Conclusions: Serum NGAL level seems to be significantly elevated in asthmatic children and can be related to asthma severity.