This study aimed to evaluate the potential activity of persimmon fruit juice (PFJ) against rheumatoid arthritis (RA). The study conducted on 35 female albino (Sprague-Dawley) rats weighing (170±10g)were randomly divided into five groups(7each) fed on basal diet the first group was the control negative and four other groups were injured with 1ml/kg of Freund's complete adjuvant into the left hind knee joint for7days to induce RA. They were re-divided as follows: group2 served as the control positive group and the three other groups were treated with different doses of PFJ (low)1ml, (high)2ml/kg bw and drugs Diclofenac sodium(DS)10mg/kg bw, respectively. The experiment lasted for 28 days. The chemical results displayed that the persimmon fruit had a high content of fiber, total phenolic, ascorbic acid content and antioxidant activity. While, the biological results revealed that the all treated groups by)low, high doses of PFJ and DS) showed a significant decrease in paw volume as well as improved biochemical and hematological analyses such as white blood cell count, erythrocyte sedimentation rate, C-reactive protein, rheumatoid factor, malondialdehyde, interleukin(IL)-6 and tumors necrosis factor(TNF)-α levels. While showed a significant increase in body weight, hemoglobin level, red blood cell count, and antioxidant enzymes in joint tissue. Histological examination of joints tissue confirmed the results of biochemical analyzes of blood. It can be recommended that the necessity consumption of persimmon fruit juice in the diets of peoples exposed to rheumatoid arthritis, because of their anti-rheumatoid arthritis and antioxidants properties, and that play important role of health status.