Background: Perceived organizational support is one of the main concepts that have a considerable impact on organization, it affects all organizational policies The study aims to examine the effect of   an educational program about organizational support on nurse's work performance Design: Quasi experimental research design used in carrying out this study. Setting: This study conducted at Nasser Institute Hospital. Subjects: Study subjects included all staff nurses who a year work (their total number is 125 staff nurses). Tools of data collection: Data in this study was collected by using three types of tools, namely work performance questionnaire & organizational support scale and knowledge regarding organizational support questionnaire. Results: The current study denoted that, more than three -fifths (62.4%) and about two-thirds (59.2%) of the studied staff nurses perceived (high work) performance at post-test and follow-up phase respectively. Also, more than two-thirds (69.6% & 67.2%) of the studied staff nurses perceived high organizational support at post-test and follow-up phase (respectively). Conclusion: Statistically significant positive correlation between work performance, knowledge, and organizational support during pre, post & three months follow up among the studied staff nurses. Recommendations: Hospital administrators and nurse administrators must provide training programs to maintain or enhance thriving at work for nurses. Implementing work life balance policies and distributing of assignment among staff nurses according to their abilities and need.