Background: Patients' safety guidelines in dialysis Settings should be applied which are Guidelines regarding patients' safety on nurses' performance in the hemodialysis unit at Benha University Hospital. Aim of the study: Was to evaluate effect of guidelines regarding patients' safety on nurses' performance in Hemodialysis Unit at Benha University Hospital. Design: Quasiexperimental research (pre/post) design was utilized to conduct the aim of this study. Setting: The
study was conducted in Hemodialysis Unit at Benha University Hospital. Sample: all available nurses (60) from both sexes who are working at the previous mentioned setting. Tools: Three tools are used; Tool I: Nurses' structured interviewing questionnaire, Tool II: An Observational checklist for patients' safety measures in dialysis unit, and Tool II: Safety Attitude Questionnaire. Results: There was statistical significant differences between nurses' knowledge 13.3% of the studied nurses had satisfactory level of total knowledge before implementation of guidelines. While improved to 90.0% after implementation of guidelines, concerning nurses' practices regarding patients' safety, 16.7% of the studied nurses were competent before implementation of guidelines, while improved to 86.7% after implementation of guidelines and 30.0% of the studied nurses had positive attitude before implementation of guidelines, while improved to 91.7% after
implementation of guidelines. Conclusions: Implementing of the guidelines had significant improvement in nurses knowledge, practices and had a positive attitude than pre guidelines implementation. Recommendations: Continuous evaluation of nurses in hemodialysis units as a method of forcing self-maturation trough education and gaining new knowledge related to their working field.