Background: The most frequent metabolic abnormality seen in newborns is hypoglycemia. A major problem in the treatment of the newborn is screening at-risk newborns and managing low blood glucose levels in the first few hours to days of life. Muscle weakness, glycogen depletion, brain energy failure and decreased glucose production are all consequences of severe glucose deprivation. The preservation of the supply of glucose to all organs is a crucial physiological process. The study aim was to: Evaluate the effect of an educational program on nurses' performance regarding care provide for neonates with hypoglycemia at neonatal intensive care units. Research design: A quasi-experimental design was utilized to conduct this study.  Settings: The current study was conducted at the neonatal intensive care unit, at Benha university hospital, Benha Specialized Pediatric. Sample: A convenient sample of all available nurses (80) working in previous mention setting& Neonates (80). Tools: Three tools were used, Tool (I): A structured interviewing questionnaire sheet including the following parts: Part (1): Personal characteristics of the studied nurses. Part (2): Personal characteristics of studied neonates Part (3):   A questioner sheet to assess nurses' knowledge Tool (II):  An observational checklist to assess nurses' practice Tool (III): A questioner to assess nurses' attitude. Results: There were a statistically significant differences in improvement nurse's knowledge, practice and attitude regarding care of neonates with hypoglycemia pre / post program implementation.  Conclusion: The educational program was effective in improving nursing knowledge, practice and attitude regarding care of neonates with hypoglycemia.  Recommendation: Emphasizing on the importance of continuing in service education for nurses regarding care of neonates with hypoglycemia to keep them up-to date in knowledge, practice and attitude.