Aim: Evaluate effect of training program on parturient women's satisfaction and nurses' compliance with natural measures for relieving labour pain. Design: A quasi- experimental (Pre/post-test). Setting: Delivery room of Obstetrics and Gynecological department at Benha University hospitals. Sampling: Two samples were used; 47 Nurse and 200 parturient women both selected by purposive sample. Tools: Three tools for nurses; a structured Interviewing Questionnaire, nurses' compliance checklist with natural measures for relief pain during labour, Nurse's satisfaction regarding implemented training program about natural pain relief measures. Four tools for parturient women; a structured Interviewing Questionnaire, Rating Visual Analog Scale, Qualitative pain assessment, Parturient woman satisfaction regarding pain relief during labour. An instructional supportive nursing brochure. Results: There was a marked improvement in all domains of compliance with natural measures for relieving pain during labour among the studied nurses at post intervention compared to pre intervention with a highly statistically significant difference (P≤ 0.001). There is a marked decrease in pain level at 1^st and 2^nd stage of Labour among breathing exercises, foot and hand, back massage and counter pressure groups at post intervention compared to pre intervention with a highly statistically significant difference (P≤ 0.001). There is no significant difference between breathing exercises, foot and hand, back massage and counter pressure groups towards satisfaction regarding pain relief during labour at post-intervention (P> 0.05). Conclusion: Training program has positive effect on parturient women's satisfaction and enhance nurses' compliance with natural measures for relieving pain during labour. Recommendation: Developing awareness program for nurses to enhance compliance and improve parturient women's satisfaction regarding natural measures for relieving pain during labour.