Background: Professional competence in nursing is a complex, relative, context dependent, and variable concept, and involves the combination of knowledge, skills, and attitudes, and the logical, scientific, and behavioral features in an individual Aim: Assess the effect of educational program about professional competence among staff nurses on their occupational personality. Design: A quasi experimental design was used. Setting: Benha University Hospital. The departments whom staff nurses were working in distributed as medical-surgical units, which were composed of bed capacity (880). Subjects: The total sample was all 295 staff nurses working in above mentioned setting. Tools: Four tools were employed in this study, 1) nurses' professional competence knowledge questionnaire, 2) nursing competence scale, 3) nurses' professional competence observational checklist, 4) occupational personality questionnaire. Results: There were a highly statistical significant positive correlation between nurses' total professional competence knowledge, perception and practice scores and their occupational personality score at post program and there was a statistical significant positive correlation in follow up program phase compared with preprogram phase. The study concluded that: Implementation of education program for professional competence was associated with significant improvement staff nurses' knowledge, perception and practice bout professional competence. which increase their occupational personality. Recommendations: Conducting training and education programs must be a continuous process for refreshing and increasing nurses' knowledge , perception and practice about the concept of professional competence and ideals models application in clinical practice of nurses.