Background: Hysteroscopy is one of the most common gynecological procedures, the most accurate technique in order to visualize the endometrial cavity, diagnose and treat the relevant pathologies. Purpose: To examine the effect of nursing interventions on maternity nurses' performance regarding the care of women undergoing hysteroscopy. Design: A quasi-experimental design was used. Setting: The Gynecological department at Menoufia University hospitals. Sample: A purposive sample containing 45 nurses for six months. Instruments: The instruments used for data collection were a structured interview questionnaire sheet, and the nurses' performance observational checklist. Results: Nurses had higher level of knowledge on post -test related to definition, indications, suitable time, complications, time needed for diagnosis, and time needed for therapeutic or surgical treatment of hysteroscopy (100%) compared to (2.2%) on pretest. Also they had higher level of performance on posttest (91.1%) than pretest (13.3%) related to pre, intra, and post-operative preparation for women undergoing hysteroscopy. The present study revealed that there was a high statistically significance difference between the studied nurses' total knowledge score and their residence, there was a high statistically significance relation between the studied nurses' total practice level regarding the care of women undergoing hysteroscopy and their socio-demographic characteristics in the residence. While there was a high statistically significant positive correlation between the total knowledge score and the total practice level of studied nurses. Conclusion: The studied nurses had poor knowledge level and low level of performance regarding the nursing care provided for women undergoing hysteroscopy procedure before introducing the nursing intervention program. But after implementing the nursing program; the studied nurses' knowledge and practice were improved. Recommendation: Continuous training programs regarding hysteroscopy for maternity nurses to develop and improve their knowledge and practice.