Abstract: Background: Nursing informatics has the potential to improve the health of individuals and the performance of providers, leading to improved quality, cost savings, and greater patient engagement in health care theirs. The implementation of nursing informatics has met with both acceptance and resistance. Purpose: determine nurses' knowledge, practice and attitude regarding   nursing informatics and its relation with their readiness for change at Menoufia University Hospital. Design: A descriptive correlational research design was used. Setting: This study was conducted in in all pediatric units in hospital at Shebin El-Kom, Menoufia Governorate, Egypt, namely: Menoufia University Hospital. Sample: All staff nurses who worked in pediatric critical care units and departments (n=300). Instruments: four instruments of data collection were used as follows: Instrument I: nurses' knowledge scale: consisted of two parts: part 1 (personal characteristic), part 2 (Nurses' knowledge scale). Instrument II: (Nurses' practice scale) .Instrument III:  (Nurses' attitude scale). Instrument IV: Readiness for change scale. Results: The majority of studied sample had poor knowledge level about nursing informatics while had good practice level about nursing informatics. Also, more than half of nurses had positive attitude regarding nursing informatics. More than half of study nurses had low level of readiness for change. Conclusion: There was highly statistical significant correlation between total knowledge level, total practice level , total attitude level   and readiness for change Recommendation: Provide periodic training programs for nurses regarding nursing informatics.