Background: Improving women's knowledge, beliefs, and practices based on wound care guidelines should be recognized as being essential to reduce post-cesarean wound infection. The purpose of the study was to assess the effect of wound care guidelines on women's knowledge, beliefs, and practices regarding cesarean section wound healing. Methods: A quasi-experimental design was utilized. Sample: A purposive sample consisted of 200 pregnant women undergoing cesarean section. Setting: The study was carried out at the Obstetrics and Gynecological Department& outpatient clinic at Beni-suef University Hospital in Beni-suef Governorate, Egypt. Instruments of this study were a structured interviewing questionnaire to assess pregnant beliefs in women knowledge and beliefs regarding wound care guidelines and self-care practice tool. Results: There was a statistically significant reduction beliefs in postoperative wound infection in the study group compared to the control group. Conclusion: Women had higher knowledge, beliefs, and practice score about wound care guidelines regarding caesarean section wound healing. Recommendations:  There is more knowledge, beliefs and practices related to cesarean wound healing that need to be corrected.